Histochemical demonstration of neurotoxic esterase.

Histochemical demonstration of desialation

Experiments were carried out to assess the susceptibility of normal and inflammatory bowel disease rectal mucus to desulphation and desialation by faecal extracts and by bacterial sialidase. The effects were assessed histochemically using a combined high iron diamine (HID) and alcian blue (AB) stain for sulphomucins and sialomucins. Rectal mucus in biopsies from controls (irritable bowel syndro...

Partial characterization of neurotoxic esterase of human placenta.

Neurotoxic esterase (NTE), the putative target for organophosphorus-induced delayed axonopathy, has been found in preparations of human placenta. The activity was primarily found in membrane-enriched fractions rather than high-speed supernatant. NTE was solubilized from a mixture of mitochondrial and microsomal membranes with Triton X-100. The crude and solubilized activities had inhibitor char...

Histochemical Demonstration of Diaphorases and Dehyd .

T HE PROBLEMS involved in the cytochemical demonstration of the activity of oxidative enzyme systems in the cells of bone marrow and peripheral blood relate mainly to methods of fixation and to the ultimate identification of the cells in which the enzyme reaction takes place. Approaches have been suggested for the demonstration of the activity of specific dehydrogenase systems in blood cells, s...

Histochemical demonstration of acetylcholinesterase in neuroblastoma.

The presence of acetylcholinesterase in the tumour cells of neuroblastoma has been shown by enzyme histochemistry. For comparison, some other tumours likely to be found in children and commonly presenting histologically as small cell tumours have also been studied. Acetylcholinesterase activity was seen in rhabdomyosarcoma, but, compared with neuroblastoma, the activity was focal and sparse. On...

Histochemical demonstration of glycogen synthesis in vitro.